Hello everyone. I’m Diego Rodriguez. I am a Masters student in the University of Illinois under the supervision of Dr. Hans Stein. And today I will talk about the effect of using two different sources of DDGS—Dakota gold and a conventional DDGS—on wean to finish growth performance and carcass quality of pigs fed diets that were provided in a pelleted or in a meal form. This is the outline of this presentation. First, I’m going to talk about Dakota gold DDGS and the nutritional values, followed by the advantages and disadvantages of pelleting diets. Then I’m going to move into the materials and methods. And after that, I will present the results of this study and draw some conclusions. Dakota Gold is a corn distillers dried grain with solubles that is produced by Poet. It is obtained by a cold fermentation process that is also called a BPX process. Dakota Gold DDGS is characterized for having a low oil concentration, and this heat free process and a low fat concentration could affect energy and amino acid digestibility and have implications in terms of growth performance. Each source of the two types of DDGS—Dakota Gold and conventional DDGS—were evaluated before we started the experiment. The first thing that we can observe here is that Dakota Gold has a lower fat concentration compared with the conventional DDGS. In a previous experiment, we determined the metabolizable energy. And we observed that Dakota Gold had lower ME compared with the conventional DDGS. This difference was around 200 kcal/kg, and this is probably because of the lower concentration of fat in Dakota Gold. The crude protein is slightly greater in Dakota Gold as well. In a previous experiment, we evaluated the amino acid digestibility and we calculated digestible amino acids of the two sources of DDGS. And we found out that concentration of digestible amino acids is greater in Dakota Gold compared with conventional DDGS. Pelleting process is a technology that is usually used in the feed industry. As a technology, it has some advantages. For example, there is an improvement in handling properties. Some authors have reported that pelleted diets may increase digestibility of nutrients, and therefore the growth performance will be improved. On the other hand, it has been reported that the heat process used at the moment of pelleting diets may damage the amino acids. It’s also good to take into consideration that pelleting diets will generate extra costs to the process. However, there is limited information about the effect of pelleting diets that contain conventional DDGS or Dakota Gold DDGS in growth performance and meat quality. Therefore, the objective of this research was to determine the effect of including Dakota Gold or a conventional DDGS in diets that were fed in a meal or a pelleted form to pigs from weaning to market phase. Let’s move on into the materials and methods. We used 160 weaned barrows and gilts. the pigs were weaned at day 21. We formulated 4 diets and we had 10 pens per diet with 4 pigs per pen. Let me explain the experimental diets. During the nursery phase, we had three different phases. During the phase 1, pigs were fed for the initial 7 days on a corn-soybean meal diet with no inclusion of DDGS. During the phase 2, pigs were fed for 14 days on diets containing corn, soybean meal, plus 15% of DDGS. During the Phase 3, pigs were fed for 22 days, and we increased to 30% inclusion of DDGS into the corn-soybean meal diets. During the grower phase, pigs were fed for 38 days, for early finishing phase pigs were fed for the 38 days, and during the late finishing phase, pigs were fed for 18 days. And all diets during growing to finisher period contained 30% of DDGS in a corn-soybean meal diet. All diets were formulated based on the metabolizable energy values and the concentration of digestible amino acids obtained in the previous experiments. We balanced the diets for amino acid by including more synthetic amino acids in diets containing the conventional DDGS because of the lower concentration of digestible amino acids in conventional DDGS. However, we did not balance the energy concentration in diets; therefore, diets containing Dakota Gold DDGS has a lower metabolizable energy compared with diets containing the conventional DDGS. The dietary treatment, we had two factors: As factor 1, the source of DDGS—Dakota Gold or conventional DDGS. And as factor 2, the diet form—in a meal or in a pelleted form. For the statistical analysis, data were analyzed the MIXED procedure of SAS. We had a 2x2 factorial treatment regimen with the DDGS source, the diet form, and the interaction between the diet form and the DDGS source. Even though we analyzed the interaction, it was not significant in our response criteria and therefore, it was eliminated in the final model. Pigs were blocked by body weight but balanced by sex. Let’s move on into the results. Because the interaction was removed from the final model, the data will be presented as two main effects, which are the source of DDGS and the diet form. Pigs were fed the diets containing DDGS during three nursery phases; however, the data will be presented for the overall nursery phase. First off, let me set up the slides. The source of DDGS will be presented with orange bars, where Dakota Gold is presented in dark orange and the conventional DDGS in light orange. Diet form will be presented with blue bars, where meal diet is presented in dark blue and pelleted diets in light blue. Average daily feed intake was not different between pigs fed the two sources of DDGS. However, pigs fed meal diets had a greater average daily feed intake than pigs fed pelleted diets. The reason for this difference may be because of more feed wastage or just because pigs ate more. Average daily gain did not differ between pigs fed diets containing the two sources of DDGS. The same story was observed that average daily gain of pigs was not affected by diet form. Gain:feed ratio did not differ between pigs fed either Dakota Gold or conventional DDGS. When we formulated diets, the metabolizable energy was not balanced, even though diets containing Dakota Gold DDGS had a lower metabolizable energy. And with this result, we assume that we probably underestimated the ME in Dakota Gold DDGS. Meanwhile, pigs fed diets in a meal form had a lower gain:feed ratio compared with pigs fed pelleted diets. That is because of the no difference in average daily gain between pigs fed meal or pelleted diets, and the higher feed intake in pigs fed the meal diets. For the final body weight, pigs did show any differences between the two sources of DDGS or between the two diet forms. On day 43, pigs were moved into a growing to finishing barn to continue the trial. Pigs stayed in the same allotment with the same treatment until the end of the experiment. There were three phases, and the phases include grower, early finisher, and late finisher. However, the data here will be presented as an overall. Average daily feed intake did not differ between pigs fed Dakota Gold or conventional DDGS. However, pigs fed diets in a meal form tend to have greater feed intake compared with pigs fed pelleted diets. These results were similar to what we observed during the nursery phase. Pigs fed the two sources of DDGS did not show any difference in average daily gain. And the same was observed between pigs fed diets in a meal or in a pelleted form. For the gain:feed ratio, pigs fed Dakota Gold or conventional DDGS did not show any difference. That, again, is telling us that the difference in ME between Dakota Gold and the conventional DDGS is not that great, to affect the gain:feed ratio during the growing to finishing phase if DDGS is included by 30% in diets. However, pigs fed pelleted diets had a greater gain:feed ratio compared with pigs fed meal diets. Now we are seeing the final body weight of the pigs, and there was no difference between pigs fed either Dakota Gold or conventional DDGS, or pigs fed diets in a meal or in a pelleted form. At the conclusion of the experiment, the pig that was closer to the average final body weight of each pen was euthanized and carcass characteristics were evaluated. The percentage of dressing in carcass did not differ between pigs fed diets containing either Dakota Gold or conventional DDGS. The same was observed that dressing percentage did not differ between pigs fed diets in a meal or in a pelleted form. The back fat was not different between pigs fed diets containing either Dakota Gold or conventional DDGs. However, pigs fed diets in a meal form had a lower back fat compared with pigs fed diets in a pelleted form. Combined with growth performance data, it appears that pigs fed pelleted diets were available to utilize more energy from the diets, which may result in more fat deposition from the excess of energy. Fat free lean was calculated with the hot carcass weight, the loin muscle area, and the back fat. There was no difference in the calculated fat free lean between pigs fed diets containing the two sources of DDGS. But as a result of greater back fat in pigs fed pelleted diets, there was a reduction in fat free lean compared with pigs fed diets in a meal form. Let me summarize what we have discussed. Even though Dakota Gold DDGS contained lower metabolizable energy compared with conventional DDGS, the growth performance was no different between pigs fed diets containing the two sources of DDGS if 30% of DDGS is included in diets. However, pigs fed pelleted diets have improved gain:feed ratio compared with pigs fed diets in a meal form. On the other hand, for carcass characteristics, there was no difference between pigs fed diets containing either Dakota Gold or the conventional DDGS. But pigs fed diets in a pelleted form had a greater back fat compared with pigs fed diets in a meal form. And with that, I would like to appreciate Poet for supporting this experiment, and our team members for supporting me with unconditional help. Thank you for listening, and if you would like to know more about this topic or about swine nutrition in general, I would encourage you to visit our website at nuturition.ansci.illinois.edu. Thank you.