Stein

An expert consensus framework for a database on ileal amino acid digestibility and protein quality scoring from foods consumed by humans.

Tome, D., J. Calvez, R. Elango,.E. Ferriolli, C. Gaudichon, G. Courtney-Martin, F. Han, M. Hayes, S. Hodgkinson, A. Kurpad, J. J. Mes, V. Owino, I. Recio, S. P. Shertukdef, H.H. Stein, A. Vlassopoulos, M. Xipsiti. 2025. An expert consensus framework for a database on ileal amino acid digestibility and protein quality scoring from foods consumed by humans. Current Dev. Nutr. 9:107291. doi.org/10.1016/j.cdnut.2025.107291 Link to abstract.

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Protein quality of salmon protein hydrolystes using the digestible indispensable amino acid score (DIAAS) method

Fanelli, N. S., J. C. F. R. Martins, and H. H. Stein. 2025. Protein quality of salmon protein hydrolystes using the digestible indispensable amino acid score (DIAAS) method. Current Dev. Nutr. 9:106016. doi.org/10.1016/j.cdnut.2025.106593 Link to abstract.

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Determination of net energy and nitrogen balance in group-housed pigs fed diets containing varying levels of starch and fiber

Lee, S. A, and H. H. Stein. 2025. Determination of net energy and nitrogen balance in group-housed pigs fed diets containing varying levels of starch and fiber. J. Anim. Sci. 103(Supplement_3), 181-182. doi.org/10.1093/jas/skaf300.221 Link to abstract.

 

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Greenhouse gas emission, nitrogen balance, and ileal digestibility of amino acids and acid hydrolyzed ether extract in diets containing different inclusion rates of hybrid rye fed to growing pigs

Acosta, J. P., S. A Lee, and H. H. Stein. 2025. Greenhouse gas emission, nitrogen balance, and ileal digestibility of amino acids and acid hydrolyzed ether extract in diets containing different inclusion rates of hybrid rye fed to growing pigs. J. Anim. Sci. 103(Suppl3), 186-187.  doi.org/10.1093/jas/skaf300.224 Link to abstract.

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Soybean meal remains a valuable pig feed ingredient

Cristobal, M., S. A. Lee, C. M. Parsons, and H. H. Stein. 2025. Soybean meal remains a valuable pig feed ingredient. National Hog Farmer, On-line edition, October 30, 2025. Link to full text.

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Effects of heat damage and crystalline amino acid supplementation on growth performance of weanling pigs fed soybean meal and enzyme-treated soybean meal

Excessive heat during processing can reduce the concentration and bioavailability of Lys in soybean ingredients via Maillard reactions, lowering growth performance in weanling pigs. Reactive Lys (the Lys not bound in Maillard products) is the relevant bioavailable part of Lys that can be used for protein synthesis by the animals. We tested the hypothesis that: (1) growth performance can be predicted from reactive Lys supplied by heat-damaged soybean ingredients, and (2) crystalline amino acids (AA) can be added to the diet to compensate for heat-induced losses.

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Choice white grease equivalence of fat emulsifier in diets fed to growing pigs

Lee, S. A., V. Perez, H. Stein. 2025. Choice white grease equivalence of fat emulsifier in diets fed to growing pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Page 384 doi.org/10.1016/j.anscip.2025.07.166 Link to abstract.

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Effect of pistachio shell powder on growth performance of weanling pigs

Kim, Y. S. A. Lee, H. Stein. 2025. Effect of pistachio shell powder on growth performance of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Page 380. doi.org/10.1016/j.anscip.2025.07.156  Link to abstract.

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Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs

Mallea, A., S.A. Lee, H. Stein. 2025. Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, August 2025, Pages 374-375 doi: 10.1016/j.anscip.2025.07.144 Link to abstract.

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Effects of dietary protein on nitrogen balance, reproductive performance, colostrum and milk compositions, and blood immune characteristics of sows

Soybean meal (SBM) is an important protein source in swine diets, but inclusion is sometimes reduced and SBM is partially replaced with synthetic amino acids (AA) because this may sometimes reduce diet costs. Over the past few decades, reproductive efficiency in sows has improved significantly, due to genetic selection, resulting in litters that can exceed 20 piglets. This increase in prolificacy has, therefore, increased the metabolic demands for AA and energy during both gestation and lactation. Adequate energy intake in gestation supports fetal growth and mammary development, but excessive energy intake during gestation may reduce feed intake during lactation, which has a negative effect on milk production. Likewise, high protein intake during gestation will improve milk production, protein accretion, and litter and pig weight at weaning, but it may also lead to an increase in fat accretion rather than protein accretion in gestating sows.

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Novel milk ingredient blend in nursery pig diets did not improve growth performance and survival compared to control diets without or with spray-dried plasma

Shen, Yanbin, Sung Woo Kim, Hans H. Stein, Javier Polo, and Joe Crenshaw. 2025. Novel milk ingredient blend in nursery pig diets did not improve growth performance and survival compared to control diets without or with spray-dried plasma. Front. Anim. Sci. 6:1648283.

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Effect of different dietary protein levels on nitrogen retention in weanling, growing, and finishing pigs

Soybean meal (SBM) is the primary plant-protein source in diets for pigs and provides both amino acids (AA) and energy to the diets. Current estimates for net energy in SBM are less than for cereal grains, based on the assumption that there is more nitrogen to be deaminated if ingredients are high in protein because deamination and excretion of nitrogen via the urea cycle are energy-requiring processes, and therefore, reduce energy efficiency. It has been suggested that pigs retain only 45 to 50% of absorbed nitrogen, which corresponds to 40 to 45% of ingested nitrogen. Modern genotypes of pigs, however, have improved the capacity for protein synthesis and may retain more nitrogen than older genotypes, which would result in less AA deamination and, therefore, less energy loss to deaminate AA and excrete nitrogen. Indeed, results of recent research indicate that pigs fed corn-SBM based diets retain more than 60% of ingested nitrogen, indicating that protein retention by modern genotypes of pigs is more efficient than by older genotypes. It is likely that as breeding companies have selected for leaner pigs, they have also selected genotypes that are more efficient in converting dietary protein into body protein. It is, however, not known if the greater nitrogen retention that has been recently reported is experienced by all pigs regardless of body weight (BW) and if it is true for all types of diets regardless of the dietary level of protein. Therefore, the objective of this experiment was to test the hypothesis that nitrogen retention, measured as a percent of nitrogen consumed, is greater than 50% regardless of the dietary protein level and the BW of pigs.

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Impact of soybean hulls on net energy and nitrogen balance in group-housed pigs allowed ad libitum access to feed

Diets for pigs may vary in composition and in their concentration of the energy containing nutrients (i.e., protein, fat, starch and sugars, and dietary fiber). To our knowledge, however, there is no information on how concentration of net energy (NE) is affected by dietary fiber concentrations in diets fed to group-housed pigs. Therefore, the objective of this experiment was to test the hypothesis that increasing soybean hulls in diets for group-housed growing pigs decreases NE and nitrogen balance.

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Amino acid quality of meat proteins. In: Preedy, V. R., editor, Plant and animal proteins in health and disease prevention.

Fanelli, N. S., and H. H. Stein. 2025. Amino acid quality of meat proteins. In: Preedy, V. R., editor, Plant and animal proteins in health and disease prevention. CRC Press, Boca Raton, FL., USA. P 71-81. doi:10.1201/9781003597926-6 Link to full text.

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Choice white grease equivalence of fat emulsifier in diets fed to growing pigs

Lee, S. A., V. Perez, H. Stein. 2025. Choice white grease equivalence of fat emulsifier in diets fed to growing pigs. Animal - Science proceedings, Volume 16, Issue 2, P. 384. Link to abstract.

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Effect of pistachio shell powder on growth performance of weanling pigs

Kim, Yeonwoo, S. A. Lee, H., Stein. 2025. Effect of pistachio shell powder on growth performance of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, P. 380. Link to abstract.

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Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs

Mallea, A., S. A. Lee, H. Stein. 2025. Effects of supplemental phytase on growth performance, blood inositol levels, and immune characteristics of weanling pigs. Animal - Science proceedings, Volume 16, Issue 2, P. 374-375. Link to abstract.

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